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KMID : 1004820080090010031
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Journal of Biomedical Research
2008 Volume.9 No. 1 p.31 ~ p.38
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Interaction of PEBP2βand TEL/RUNX1 proteins
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Lee Young-Ju
Kim Jae-Hyun
Park Mi-Na
Choe Soo-Young
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Abstract
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Acute lymphoblastic leukemia (ALL), a predominantly pediatric disease involving uncontrolled proliferation of white blood cells within the bone marrow, is strongly associated with chromosomal translocations. The human chromosomal translocation t(12;21)(p12;q22) is the most frequently encountered chromosomal rearrangement in pediatric B-lineage ALL , and results in fusion of the TEL and RUNX1 genes. The resulting TEL/RUNX1 fusion protein is generally thought to be a transcriptional repressor that interferes with RUNX1-mediated transactivation.We used a luciferase assay system to investigate the effects of TEL/RUNX1 and PEBP2βon RUNX1-mediated transactivation. TEL/RUNX1 blocked the synergistic transactivation achieved by PEBP2βand RUNX1, and coimmunoprecipitation and immunofluorescence analyses showed that PEBP2βinteracted with TEL/RUNX1 and was sequestered in the cytoplasm. These results suggest that TEL/RUNX1 inhibits RUNX1-mediated transactivation via cytoplasmic sequestration of coactivator(s) such as PEBP2β.
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KEYWORD
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TEL/RUNX1 fusion protein, PEBP2βtranscription, leukemia
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